Raturinarybladderurothelialcells
1. Bladders were excised from deeply anesthetized (urethane, 1.2 gm ? kg?1, i.p.) Sprague Dawley rats (of either sex), cut open, and gently stretched (urothelial side down). 2. Anesthesia was determined to be adequate for surgery by periodically testing for the absence of a withdrawal reflex to a strong pinch of a hind paw and absence of an eye-blink reflex to tactile stimulation of the cornea. 3.......閱讀全文
Rat－urinary－bladder－urothelial－cells
1.?Bladders were excised from deeply anesthetized (urethane, 1.2 gm???kg?1, i.p.) Sprague Dawley rats (of either sex), cut open, and gently stretc
Culturing－Rat－Fetal－Neural－Stem－Cells
實驗概要Rat ?neural stem cells (NSCs) serve as a well-established model for ?investigating human brain development, disease processes, and treatment ?stra
Isolation－and－characterization－of－rat－glomerular－endothelial－cells
Glomerular endothelial cells (GECs) from the kidney are in close juxtaposition to other cell types, such as mesangial cells, and may respond to as
Isolation－and－culture－of－rat－coronary－microvascular－endothelial－cells
CMVE were isolated from Wistar rat hearts by digestion of primary cell isolation kit.1.?Hearts mounted on a Langendorff apparatus were perfused at 3
Sleeping－Beauty－transposon－mutagenesis－in－rat－spermatogonial－stem－cells
Sleeping Beauty transposon mutagenesis in rat spermatogonial stem cellsZoltán Ivics,1, 2 Zsuzsanna Izsvák,1, 2 Gerardo Medrano,3, 4 Karen M Chapman3,
ThymidineIncorporation－Assay－for－Rat1a－cells
Thymidine-Incorporation Assay for Rat-1a cells??????Overview?? This method of Peter Coward, Ph.D. in the Conklin Lab was used in Coward, et al (1998)
Isolation－and－culture－of－SpragueDawley－rat－aortic－smooth－muscle－cells
The intact mature arterial media is composed of at least four phenotypically unique cell subpopulations that reside in distinct medial layers.? Th
[3H]ThymidineIncorporation－Assay－for－Rat1a－cells
Overview?This method of Peter Coward, Ph.D. in the Conklin Lab was used in Coward, et al (1998) Controlling signaling with a specifically designed Gi-
A－primary－cell－culture－model－of－rabbit－uroepithelium
Isolation of Epithelial Cells from Rabbit Bladders?1.?Animal experiments were performed in accordance with the Animal Use and Care Committee.?2.?Urina
尿液診斷膀胱癌的研究進展（二）
3.2 細胞成分檢測?3.2.1 生存素( survivin) 生存素是凋亡抑制蛋白,在多種人類腫瘤中均可上調。研究發現尿液標本中生存素水平檢測總的敏感度、特異度、陽性和陰性預測值分別為64%、93%、92%和67% [16],這些指標均優于傳統的尿液細胞學檢查和NMP22檢測,且尿液中
Global－urinary－metabolic－profiling－procedures－using－gas－chromatography
Global urinary metabolic profiling procedures using gas chromatography–mass spectrometryEric Chun Yong Chan,1 Kishore Kumar Pasikanti1, 2 & Jeremy K N
Rat－Lung－Perfusion
實驗概要The procedure presented below describes a method for perfusing rat lung.主要試劑Phosphate Buffered Saline主要設備1.Dissecting Microscope or Eye Magnifier2
Rat－Liver－Preparation
實驗概要The procedure presented below describes a method for preparing rat liver.主要試劑1.????? Aluminum Foil2.????? Liquid Nitrogen3.????? Dry Ice4.????? Ph
膀胱癌研究體內外模型綜述（一）
膀胱癌是泌尿生殖系統最常見的惡性腫瘤之一，在我國其發病率及病死率均列泌尿生殖系統腫瘤的首位。 2015年全國新確診為膀胱癌的患者人數超過8萬，導致的死亡人數超過3萬，約70%以上首診時是淺表性癌。膀胱癌的高復發率和治療后期的并發癥是臨床治療的兩大難題。深入研究膀胱癌的發病機制、發展過程及轉移規律
Rat－Blood－Collection－Protocols
實驗概要The procedure presented below describes a method for collecting rat blood.實驗步驟Rat should be fully anesthetized (e.g., unresponsive to toe pinch).1
Differentiate－ES－cells－into－glial－cells－and－neurons
Day -1: Pass ES cells at normal density on gelatinized plate to free the culture of contamination fibroblast cells.___________________Day 1:?Trypsiniz
Culture－of－BEND－Cells－(Bovine－Endometrial－Cells)
Culture of BEND Cells (Bovine Endometrial Cells)Charles E. Krininger, III and Peter J. Hansen?Dept. of Animal Sciences, University of FloridaThis prot
科學家發現膀胱癌干細胞起源的重要遺傳學機制
腫瘤干細胞在腫瘤細胞的發生、自我更新、耐藥性和轉移中扮演著重要角色。作為泌尿系統最常見的惡性腫瘤——膀胱癌干細胞（Bladder Cancer Stem Cells）的起源和遺傳學基礎目前仍不明確。 中國科學院生物物理研究所蛋白質科學研究平臺抗體工程實驗室李翀與中國科學院微生物研究所、深圳大學
膀胱癌研究體內外模型綜述
膀胱癌是泌尿生殖系統最常見的惡性腫瘤之一，在我國其發病率及病死率均列泌尿生殖系統腫瘤的首位。 2015年全國新確診為膀胱癌的患者人數超過8萬，導致的死亡人數超過3萬，約70%以上首診時是淺表性癌。膀胱癌的高復發率和治療后期的并發癥是臨床治療的兩大難題。深入研究膀胱癌的發病機制、發展過程及轉移規律
Differentiating－Neural－Stem－Cells－into－Neurons－and－Glial－Cells
實驗概要The protocols in ?this section describe the steps involved in differentiating neural stem ?cells (NSC) to neurons, astrocytes, and oligodendrocyte
RAT/MOUSE－GROWTH－HO...
實驗概要This ?Rat/Mouse Growth Hormone ELISA kit is used for the non-radioactive ?quantification of Growth Hormone in rat or mouse serum, plasma, tissue ?
Preparation－of－Rat－Liver－Cell－Cytosol
These protocols should yield enough cytosol and organelles for 1-200 MT/Organelle motility assays.Solutions and Reagents??Freshly removed or flash fro
Isolation－of－rat－cardiac－fibroblasts－and－cardiomyocytes
1.?Hearts were removed from newborn rats (day 0), put into calcium- and bicarbonate-free HEPES-buffered Hanks’ medium, cut into pieces and digeste
Preparation－of－Mitochondria－from－Rat－Liver
Preparation of Mitochondria from Rat LiverRat liver is an ideal source for functional intact mitochondria for a number of reasons. We use Sprague-Dawl
Histopathological－Approach－to－Rat－Liver－Tissue
ProcedureAfter deep ether anesthesia, dissect the rat’s liver (Wistar albino rats, 200 – 250 g) by cutting on the ventral side.Fix 2 – 3 mm. of the li
Immunofluorescent－Staining－of－Mouse－and－Rat－Leukocytes
I. ProcedureHarvest cells from tissue, preparing a single cell suspension. Red blood cells may be removed by lysis or density gradient: Red blood cell
Preparing－Mitochondria－from－Rat－Liver
Liver is a convenient source for functional intact mitochondria for a number of reasons. Animal tissue is more readily homogenized than plant tissue b
Freezing－Cells
1) Keep prepared solutions on ice.2) Determine total cell count of cells to be frozen. (e.g. 1 X 108 )3) Determine number of vials to be frozen. (e.g.
Growing－cells
No two cell lines behave exactly the same, so you must learn the peculiarities, or personality, of each of the cell lines with which you work. Irrespe
Trypsinizing－cells
There are many procedures with which to trypsinize cells. All include washing the cell monolayer with TD, or in rare cases, with VE. This removes seru